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Journal: Cancer research
Article Title: Pharmacological targeting of androgen receptor elicits context-specific effects in estrogen receptor-positive breast cancer
doi: 10.1158/0008-5472.CAN-22-1016
Figure Lengend Snippet: Relative AR/ER expression in primary and metastatic ER+ breast cancers. A, Bar chart of the percentage of AR staining intensity in ER weak (+), intermediate (2+), and strong (3+) tumors in IHC cohort. The intensity of nuclei immunoreactivity was assessed semiquantitatively on a scale of 0 to 3 (none, weak, intermediate, and strong). B, Distribution of AR/ER in IHC cohort (n = 68). Expressions were quantified using IHC scores, positive cell percentage × intensity. C, Distribution of AR/ER in TCGA breast cancer cohort (n = 347). The reverse phase protein array was used to test AR and ER protein levels. D, Violin plot of AR/ER level in primary and metastatic breast cancer. Bars are presented as 25th percentile (bottom quartile), median, and 75th percentile (top quartile). AR/ER is higher in metastatic cancer than in primary cancer (primary vs. metastatic, median 0.047 vs. 0.689, unpaired two-tailed Student t test, P = 0.0001). E and F, Kaplan–Meier survival curves displaying the estimated survival probability for two different groups (AR/ER high vs. low) of metastatic breast cancer. Patients with metastatic breast cancer (n = 97) subsequently treated with endocrine therapy were included in the analysis. Patients with AR/ER-high or -low groups were separated by median of AR/ER level in the cohort. The number of events (total deaths in each group) is indicated. Patients with lower AR/ER levels have a more favorable prognosis compared with patients who have higher AR/ER levels. E, Progression-free survival. F, Overall survival. The log-rank test indicates a significant difference between the survival curves.
Article Snippet: Recombinant full-length human AR and
Techniques: Expressing, Staining, Protein Array, Two Tailed Test